염화비닐 노출 근로자의 시료채취시기에 따른 요중 thiodiglycolic acid의 농도 변화
Urinary Excretion of Thiodiglycolic Acid According to Sampling Time in Workers Exposed to Vinyl Chloride Monomer
페이지 수 138 ~ 145
주제어 : Vinyl chloride monomer(VCM), Thiodiglycolic acid(TDGA), Sampling time, Biomarker
저자 : 김현수, 김치년, 원종욱, 차봉석, 이경종, 노재훈
Objectives: The study was performed to investigate the changes of urinary thiodiglycolic acid (TDGA) concentration in workers exposed to vinyl chloride monomer (VCM) according to the time of sampling urine. Methods: The personal exposure to airborne VCM was assessed and urinary TDGA concentration was sampled in 31 workers employed in a VCM and polyvinyl chloride (PVC) manufacturing factory. Urinary TDGA was sampled three times: before the start of the shift after 3 days off (TDGA1), after the end of the first-day shift (TDGA2) and before starting the following day shift after completing the one-day shift (TDGA3). Urinary TDGA in 30 workers who had not been exposed to airborne VCM was sampled after the end of the shift. A gas chromatography/pulsed flame photometric detector (GC/PFPD) was utilized to analyze TDGA concentration in urine after the urine was methylated with trimethylsilyldia-zomethane (2.0M in diethyl ether). Results: The creatinine level was 0.179±0.271 ㎎/g in the control workers and 0.218±0.443 ㎎/g in the workers before the start of the shift after 3 days off (TDGA1), showing no significant difference (p=0.7035). Urine samples were compared according to sampling time in order to investigate the change of urinary TDGA concentration in the case of continuous exposure to airborne VCM. In VCM-exposed workers, urinary creatinine concentration was 0.434±0.623 mg/g in TDGA2 and 0.767±1.056 ㎎/g in TDGA3, which indicated a gradual but significant increase (p=0.024). In terms of the statistical correlation between airborne VCM and urinary TDGA to evaluate exposure dose per day, of the three urinary TDGA concentrations, TDGA3 showed the highest degree of regression (R2=0.4215) with 8h-TWA airborne VCM concentration. Conclusion: Based on this result, the excretion half-life of urinary TDGA was assumed to be less than 3 days, because the concentration of urinary TDGA at 3 days after exposure to airborne VCM was decreased to the level of urinary TDGA concentration in the control workers. The concentration of urinary TDGA increased in the case of continuous shift, due to the accumulation of residual metabolites of TDGA. It was considered that TDGA3 can be applied as a useful biological index to evaluate the exposure dose of airborne VCM during one day because TDGA3 showed the highest correlation with the exposure dose of airborne VCM in the previous shift day.