Objectives: This study was performed to determine the self-perceived fatigue and its association with job stress and psychosocial factors among white collar male workers. Methods: The self-administered questionnaires were given to 872 workers employed in 42 work places located in Daejeon City and Chungnam Province during the period from February 1st to April 30th, 2009. The questionnaire surveys included socio-demographic characteristics, job-related characteristics, health-related behaviors, a Job Content Questionnaire (JCQ), psychosocial factors (type A behavior pattern, locus of control, self-esteem) and the Multidimensional Fatigue Scale (MFS). Results: Concerning the correlation of self-perceived fatigue with various factors, while the level of self-perceived fatigue was positively correlated with the job demands, a type A behavior pattern and selfesteem, it was negatively correlated with job autonomy and supervisor support. With the analysis of the covariance structure, JCQ (job demand, job autonomy, supervisor support and coworker support) had more of an influence on the level of self-perceived fatigue than the psychosocial factors (type A behavior pattern, locus of control, self-esteem). The inter-relational effects were that the higher the JCQ and psychosocial factors, the higher the level of self-perceived fatigue. Conclusions: The study results indicated that the level of self-perceived fatigue was more influenced by the JCQ than the psychosocial factors. Thus, an effective strategy for fatigue reduction among workers requires additional programs that will focus on innovated job specifications and managed psychosocial factors.